Ever wondered how the Carboxygenator works within the Osilaris™?
We get that question often, so today we’re excited to share the science behind it, step by step:
The Carboxygenator system:
𝗣𝗿𝗲𝗰𝗶𝘀𝗲 𝗚𝗮𝘀 𝗖𝗼𝗻𝘁𝗿𝗼𝗹: The Carboxygenator is a patented oxygenation system that’s all about maintaining the ideal gaseous concentrations in the culture media of the Osilaris bioreactor.
𝗜𝗻𝘁𝗲𝗴𝗿𝗮𝘁𝗲𝗱 𝗦𝗲𝗻𝘀𝗼𝗿𝘀: Oxygen and pH levels are continuously monitored with in-line optical sensors embedded within the Osilaris Adherent and Suspension culture bags.
𝗗𝗮𝘁𝗮 𝗧𝗿𝗮𝗻𝘀𝗺𝗶𝘀𝘀𝗶𝗼𝗻: As we continue in the process, this data is transmitted to the controller software of the Osilaris cabinet through transmitters built into the rocking expansion platform.
𝗔𝘂𝘁𝗼𝗺𝗮𝘁𝗲𝗱 𝗖𝗼𝗻𝘁𝗿𝗼𝗹: Our controller software takes the wheel from here. It ensures that the pre-defined culture environment is maintained by controlling the addition of gases, thanks to the data received.
𝗚𝗮𝘀 𝗜𝗻𝘁𝗿𝗼𝗱𝘂𝗰𝘁𝗶𝗼𝗻: The addition of gases takes place through gas-permeable tubing in the perfusion loop. This crucial step ensures a seamless and precise process for maintaining the optimal gas environment within the culture bag.
𝗖𝗼𝗻𝘁𝗶𝗻𝘂𝗼𝘂𝘀 𝗙𝗲𝗲𝗱𝗯𝗮𝗰𝗸-𝗟𝗼𝗼𝗽: A continuous feedback loop between the culture bag and the Carboxygenator guarantees that setpoints are maintained within minimal bandwidths.
𝗡𝗼 𝗛𝗲𝗮𝗱𝘀𝗽𝗮𝗰𝗲 𝗥𝗲𝗾𝘂𝗶𝗿𝗲𝗱: With our Carboxygenator, we can provide your cells with the oxygen they need without the use of a headspace, resulting in a homogenized culture.
Minimizing Shear Stress: What’s more, this process minimizes any additional shear on your cells as there is no air-liquid interface during the rocking process.
At Scinus, we’re proud to deliver innovation that empowers your research and cell culture experiments. If you’re as excited about this as we are, let’s keep the conversation going. Reach out, and let’s explore the possibilities together!
ATMP manufacturing the easy way:
The semi-permeable part of the tubing of the culture bag can easily be placed in the meandering channel of the Carboxygenator, after which the top plate is put on to keep the system closed and under the right pressure:
