Flexible Cell Expansion Applications for Adherent and Suspension Cultures with Osilaris™
Osilaris™ is a flexible, automated bioreactor platform supporting a wide range of clinical grade cell expansion workflows.
Using two types of expandable culture bags, the system enables scalable, closed-system expansion across multiple cell types, including MSC, MSC-EV, HEK,
T-cells (CAR-T, TCR-T, TIL), and iPSC among others, making Osilaris™ suitable for diverse cell therapy and regenerative medicine applications .
Mesenchymal Stromal Cells
Osilaris™ is well suited for adherent cell culture, including mesenchymal stromal/stem cells (MSCs), using microcarriers for scalable and efficient expansion. One Osilaris™ adherent bag holds microcarriers providing a surface area equivalent to approximately 220 T175 flasks, enabling high-density culture in a compact setup. Proven protocols support large-fold expansion of various adherent cell types, including bone marrow-derived MSCs, adipose-derived stem cells, and HEK293T cells. Also, the system is highly suitable for MSC-derived extracellular vesicle production. The closed, automated workflow reduces manual handling and contamination risk, making Osilaris™ suitable for research, process development, and GMP-aligned manufacturing.
Immune Cells
Osilaris™ enables robust suspension cell culture expansion, supporting immune cells such as T cells and Tumor Infiltrating Lymphocytes (TILs). The innovative roller-clamp system allows precise control of bag size, ensuring optimal cell concentrations in suspension throughout the culture process. Standardized protocols provide efficient, scalable, and fully closed-system operation. In addition, Osilaris™ integrates smoothly with third-party technologies, enabling seamless connection of upstream and downstream steps for a fully integrated cell therapy manufacturing workflow.
Organoids
Osilaris™ also supports rapid and scalable production of organoids in a dynamic culture environment. Controlled agitation and optimized culture conditions enable high-yield organoid expansion while preserving viability and phenotype. Demonstrated results include high-fold expansion of intrahepatic cholangiocyte organoids, underscoring the system’s suitability for both organoid-based research and therapeutic applications.
iPSCs
Osilaris™ enables efficient scale-up of iPSCs from static culture to self-aggregating spheroids, with controlled agitation and culture conditions supporting robust growth and downstream differentiation workflows. These spheroids can be directly differentiated into various lineages, making the system ideal for large-scale iPSC applications. The advanced agitation control of Osilaris™ is key in controlling spheroid formation, spheroid size, and cell viability, ensuring homogeneity, nutrient availability, and robust growth until harvest.