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Scalable MSC expansion with Osilaris™

Expand MSCs from low starting cell numbers in a closed, controlled microcarrier-based process.

Expand MSCs from low starting cell numbers to clinically relevant yields in a single, closed culture system. Osilaris™ combines microcarrier-based expansion with an expandable culture volume, low-shear mixing and integrated pH and dissolved oxygen control, including hypoxic conditions.

Demonstrated processes have achieved yields of 1–2 billion MSCs per run and more than 2,000-fold expansion. With options for closed harvest and MSC-derived extracellular vesicle (MSC-EV) production, Osilaris supports process development and scale-up towards GMP manufacturing.

Find out more about the Osilaris bioreactor

Osilaris Adherent Culture Bag

Scale within one culture bag – How increasing culture volume and microcarrier surface area supports expansion from small inoculum.

Osilaris' volume-expandable adherent culture bags enable cell-density control and more efficient MSC culture and harvesting at scale.

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Osilaris bioreactor

Control the culture environment – What pH and dissolved oxygen control including hypoxic conditions allow users to manage.

Dissolved Oxygen (DO) is controlled via the Carboxygenator, including the possibility to maintain stable hypoxic conditions. 

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Osilaris bioreactor and Rotea

Connect your manufacturing workflow – How closed connections support integration with upstream and downstream equipment.

The full process is simple by design. The closed workflow minimizes risk, and automation adds robustness. Universal tygon tubing is compatible with any third party USP/DSP.

Explore the harvest process

From flask-based culture to scalable MSC manufacturing

Osilaris MSC workflow

Scaling a flask-based MSC process often means adding more culture vessels, incubator space and manual handling. Moving to a controlled bioreactor process can simplify this workflow and reduce reliance on repeated transfers and parallel culture vessels.

Osilaris™ supports expansion from a small starting inoculum by progressively increasing culture volume and microcarrier surface area within the same bag. Low-shear mixing and integrated pH and dissolved oxygen control help manage culture conditions throughout expansion, including the option to maintain hypoxic conditions for MSC and MSC-derived extracellular vesicle (MSC-EV) workflows.

Closed harvesting and connections to compatible upstream and downstream equipment support an integrated manufacturing workflow. Flexibility in media, microcarriers and reagents allows the process to be adapted to your cells and manufacturing requirements, with Scinus experts supporting process transfer and optimization.

Culturing MSCs within the Osilaris adherent culture bag:

The Innovation Behind Our Platform

We are working with many cell therapy experts around the world and collaborate in many different projects advancing the field with the Osilaris Platform. 

Learn more about our work

"Scinus' bioreactor is the only system that offers a process compatible with patient-specific expansion of periosteum-derived cells for large bone defects"

We evaluated different and industry standard fixed-bed systems in the past, but the Scinus Cell Expansion platform has proven superior and now represents our gold standard technology. What sets the Scinus system apart is its remarkably low medium requirements, achieving unprecedented-fold expansion in a single, volume-expandable passage. The entire operation can be performed by one person, thanks to the system's intuitive handling and streamlined cell harvesting process.

Prof. Liesbet Geris

Prometheus Division of Skeletal Tissue Engineering  KU Leuven

"Scinus bioreactors have been a key investment toward our future manufacturing goals. "

The system addresses many next-generation requirements, including a closed setup compatible with tube welding, integrated sensors, low shear stress, bubble-free gas exchange, and an integrated seed train within a single expandable consumable. Early on, we also benefited from established hMSC protocols and Scinus’ process development support for new cell types. As early adopters, working with multiple system versions, we continue to grow with Scinus and value their ongoing support and expertise. The team’s knowledge and responsiveness have been instrumental, and we look forward to further integrating the platform into our automated processes. 

Dr. Ravi Sinha

Technological Manager, ReGEN Biomedical

Planning to scale your MSC process?

Do you want to discuss your current protocols and project, or just understand better how to culture MSC or other adherent cells with the Osilaris platform? Talk with one of our experts

    support@scinus.com

   +31 (0) 85 050 9300
Discuss your MSC process
Which MSC tissue sources have been successfully cultured in the Osilaris bioreactor?What microcarriers should be used for an MSC culture?How do you transfer a flask-based process into the Osilaris?Explore all FAQs here

Which MSC tissue sources have been successfully cultured in the Osilaris bioreactor?

We have successfully expanded mesenchymal stromal cells (MSCs) isolated from bone marrow, adipose tissue, and Wharton’s jelly.

Although donor- and source-specific biology can significantly affect cell attachment, growth, metabolism, and harvest, our most robust and extensively validated process uses bone marrow–derived MSCs. We recommend conducting a source-specific feasibility study before committing to expected yields or run durations.  

What microcarriers should be used for an MSC culture?

We have successfully cultured MSCs from multiple tissue sources using research-use-only (RUO) and GMP-grade microcarriers with diverse surface chemistries from several suppliers. Conventional collagen-coated polystyrene microcarriers support MSC expansion and closed-system harvesting. Dissolvable microcarriers can further simplify downstream processing; however, the best microcarrier brand and chemistries depend on the exact application and manufacturing requirements.


How do you transfer a flask-based process into the Osilaris?

A 2D protocol can provide a useful starting point. Surface area–normalized seeding density, attachment time, mixing, gas control, feeding strategy, and harvest conditions must be adapted to the bag format and selected microcarrier. We typically first perform a  small-scale microcarrier screening or spinner-flask study before conducting a confirmation run in the Osilaris. We have successfully transferred multiple MSC- and MSC-EV–based protocols from 2D culture to the Osilaris. Our Process Development team can help you choose the right microcarrier and culture conditions.


Explore all FAQs here